FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13894565
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC179650.083.051285120
GAGCACA303900.050.133358120
AGAGCAC409050.037.999954120
AAGAGCA607150.025.716812120
GAAGAGC692000.023.373709120
GGAAGAG847450.018.820705118-119
CGGAAGA960200.016.78587118-119
TCGGAAG1003800.015.847307116-117
ATCGGAA1172350.013.745258116-117
GATCGGA1201700.013.270327114-115
AGATCGG1315650.012.235492114-115
TAGATCG261550.012.137839120
AAGATCG587350.010.334237112-113
CTAGATC83550.09.981097118-119
TTAGATC115150.09.798506120
GAGATCG422600.09.710797112-113
CAGATCG377200.08.769425114-115
TAAGATC251200.08.620167116-117
CAAGATC321700.07.6895413116-117
TGAGATC208950.07.564043108-109
Produced by FastQC (version 0.11.5)