FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15519485
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG92750.011.643417120
CGTATCA371250.010.084166120
GGTCGCC598650.09.951719120
GCCGTAT461200.09.938616120
TGGTCGC632100.09.785765120
CGCCGTA489100.09.653814120
GTGGTCG698650.09.506263120
AAAAAAA811600.09.491718120
GTCGCCG591450.09.241069120
GGTGGTC780150.09.220679120
TCGCCGT572200.09.205948120
TCATTAA301500.09.074013120
CCGTATC441950.09.000408120
GTATCAT393200.08.788826120
ATCTCGG1112100.08.464493120
TCGGTGG916700.08.429668120
CTCGGTG983750.08.324723120
CGGTGGT862600.08.193278120
ATCATTA359850.08.019465120
TCTCGGT1033650.07.864799120
Produced by FastQC (version 0.11.5)