FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15519485
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC210250.074.973755120
GAGCACA331550.047.414154120
AGAGCAC445550.037.84421120
AAGAGCA668350.024.75556118-119
GAAGAGC736800.022.489822118-119
GGAAGAG908950.018.128275116-117
CGGAAGA1055150.015.857958116-117
TCGGAAG1051750.015.636829114-115
ACACGTC57800.013.6608515120
ATCGGAA1251300.013.38124114-115
GATCGGA1268350.013.094255112-113
CACGTCT58850.012.563297120
AGATCGG1387300.012.116417112-113
CACACGT56650.011.910798120
TAGATCG237600.011.239497112-113
CTAGATC68250.010.255057114-115
ACGTCTG78500.010.241446120
AAGATCG667700.010.176294110-111
GAGATCG436000.09.311006110-111
CAGATCG436650.08.918717114-115
Produced by FastQC (version 0.11.5)