FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15222117
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC184250.075.74817120
GAGCACA288200.047.440502120
AGAGCAC386600.035.91707120
AAGAGCA587950.023.740187118-119
GAAGAGC648850.021.321646116-117
GGAAGAG801750.017.419146116-117
CGGAAGA914650.015.234515114-115
TCGGAAG911200.015.226013114-115
ATCGGAA1081400.012.991105112-113
GATCGGA1106800.012.648098112-113
CACGTCT57800.011.925651120
AGATCGG1196250.011.756103110-111
CACACGT50500.011.168261118-119
TAGATCG297350.010.952831110-111
ACACGTC59350.010.776058120
CTAGATC70050.010.432173108-109
ACGTCTG76050.09.811338120
GAGATCG456300.08.723566110-111
CAGATCG467450.08.408952112-113
TTAGATC107150.08.375848116-117
Produced by FastQC (version 0.11.5)