FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15231926
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC202350.080.74913120
GAGCACA331850.051.589592120
AGAGCAC452150.038.641037120
AAGAGCA673150.025.951265118-119
GAAGAGC765250.022.886288120
GGAAGAG923450.019.140657120
CGGAAGA1098450.016.142462120
TCGGAAG1119900.015.777481118-119
ATCGGAA1319550.013.522927120
GATCGGA1357350.013.363887120
AGATCGG1502600.012.287295120
CTAGATC98100.011.037143120
TAGATCG349500.010.988293114-115
TTAGATC124400.010.568812120
ACACGTC54950.010.363877120
GAGATCG607500.010.130378118-119
CACACGT48000.09.508064118-119
CCTAGAT35100.09.301775116-117
AAGATCG425650.09.12205116-117
CAGATCG568400.09.023731118-119
Produced by FastQC (version 0.11.5)