FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15268859
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC158000.066.00611120
GAGCACA243400.044.75791120
AGAGCAC328250.033.148438120
AAGAGCA527200.020.991976118-119
GAAGAGC558100.020.095087120
GGAAGAG699100.016.126486120
CGGAAGA815700.013.884091118-119
TCGGAAG803300.013.822612120
ATCGGAA968050.011.889734120
GATCGGA962250.011.721988118-119
AGATCGG1043800.010.920188120
TAGATCG278000.010.580342120
CTAGATC68850.09.609828120
AAGATCG437800.09.142472120
GAGATCG363700.08.843748120
TTAGATC131300.08.381905120
CACGTCT49100.07.471447120
CAGATCG323950.07.3607416120
CACACGT40400.06.648148120
ACACGTC44000.06.5508575120
Produced by FastQC (version 0.11.5)