FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14828765
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC186800.085.673645120
GAGCACA299550.049.521587120
AGAGCAC416750.039.157795120
AAGAGCA640100.024.568104118-119
GAAGAGC715500.022.316786118-119
GGAAGAG858350.018.326012116-117
CGGAAGA987750.016.273224120
TCGGAAG1019350.015.452656114-115
ATCGGAA1188800.013.609439114-115
GATCGGA1235000.013.123036120
CACACGT45000.012.606961120
AGATCGG1368100.011.874968112-113
TAGATCG354700.011.087778110-111
CACGTCT51900.010.526043120
ACACGTC52450.010.282131120
CTAGATC90200.010.094685110-111
ACGTCTG67000.09.7217865120
AAGATCG439200.09.344869120
GAGATCG500850.09.158831118-119
TTAGATC148900.08.819493114-115
Produced by FastQC (version 0.11.5)