FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11528679
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC168700.0103.03314120
GAGCACA286900.061.499752120
AGAGCAC390250.044.86782118-119
AAGAGCA557500.031.270721118-119
GAAGAGC653450.026.815542116-117
GGAAGAG775650.022.454365116-117
CGGAAGA909600.019.29203114-115
TCGGAAG931150.018.513487114-115
CACGTCT47100.018.343996120
ATCGGAA1069050.016.584795112-113
GATCGGA1104950.015.754625112-113
ACACGTC53250.014.952814120
AGATCGG1204900.014.675968110-111
CACACGT46800.014.208924118-119
TAGATCG267050.013.623915110-111
CTAGATC45400.012.313801108-109
TCTAGAT24250.011.876319120
CGTCTGA77150.011.528373120
GAGATCG539400.011.432777110-111
ACGTCTG60900.011.266917118-119
Produced by FastQC (version 0.11.5)