FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16781980
Sequences flagged as poor quality0
Sequence length52-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG77300.011.022387120
TAGATCT309900.010.90068120
GTGTAGA468100.010.459678120
AGTGTAG468850.010.366159120
GAGTGTA522200.010.295275120
AGATCTC302700.010.089559120
AGAGTGT583650.010.05433120
GTAGATC468350.09.77509120
GATCTCG299400.09.639623120
ATCTCGG287200.09.610372120
AAGAGTG685850.09.544703120
GTAGGGA1029900.08.558418120
TGTAGAT521450.08.538037120
TCTCGGT268950.08.4777155120
TAGGGAA981350.08.413201120
TGTAGGG1106350.08.379204120
GGGAAAG927500.08.364719120
GTGGTCG157950.08.319386120
TCGGTGG234950.08.274397120
GTGTAGG1212200.08.157362120
Produced by FastQC (version 0.11.5)