FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11967418
Sequences flagged as poor quality0
Sequence length54-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT72700.012.047815120
GAGTGTA119250.011.067617120
GTAGGGA272250.010.599052120
AGTGTAG108200.010.03555120
AGAGTGT136900.09.991283120
AAGAGTG168150.09.81128120
TGTAGGG306600.09.783352120
TAGGGAA249750.09.728354120
CGTGTAG354150.09.452285120
GTAGATC131500.09.443532120
GTGTAGA118450.09.065835120
GTGTAGG332600.08.874271120
GTCGTGT403150.08.496877120
TCGTGTA376300.08.290085118-119
CGTCGTG480600.08.088744120
AAAGAGT217400.07.643813120
GAGCGTC594100.07.3714542120
AGGGAAA334350.07.302691120
AGCGTCG548750.07.237246120
GCGTCGT516350.07.215016120
Produced by FastQC (version 0.11.5)