FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11967418
Sequences flagged as poor quality0
Sequence length50-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC94300.042.335213120
GAGCACA140100.031.578405120
AGAGCAC174700.025.28886120
AAGAGCA284350.016.209757120
GAAGAGC312100.015.677926120
GGAAGAG368300.013.000772120
GATCGGA454700.012.905199120
TCGGAAG379300.012.656277120
CTAGATC34100.012.485441120
ATCGGAA464700.011.2864120
CGGAAGA432550.011.240847120
AGATCGG532350.011.115539120
TAGATCG121450.010.440576118-119
TTAGATC49500.08.102504116-117
GAGATCG272250.07.1392527118-119
CACACGT27700.06.9054356120
AAGATCG224250.06.7550597118-119
CCTAGAT16500.06.5443296116-117
AGAGATC91750.06.1871543120
CAGATCG253750.06.127787120
Produced by FastQC (version 0.11.5)