FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13739070
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC163350.074.85333120
GAGCACA257100.048.41445120
AGAGCAC356650.035.594917120
AAGAGCA533600.023.894615118-119
GAAGAGC602800.021.367958120
GGAAGAG754900.017.618362120
CGGAAGA891100.014.891022118-119
TCGGAAG894450.014.827559118-119
ATCGGAA1047100.012.699174116-117
GATCGGA1065850.012.542921120
AGATCGG1175900.011.550353120
CACACGT46100.011.33734120
CTAGATC67700.010.767521120
TAGATCG270850.010.638767118-119
ACACGTC55500.010.160601120
TTAGATC102150.09.99787116-117
AAGATCG503350.09.632025120
CCTAGAT21900.09.265071112-113
CACGTCT52700.09.004032120
ACGTCTG68750.08.802549120
Produced by FastQC (version 0.11.5)