FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15327478
Sequences flagged as poor quality0
Sequence length50-126
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG66750.011.953728120
TCCGATC67650.010.9081983
CCGATCT64850.010.82381254
CGATCTA16500.010.5443175
GGTCGCC610450.09.179092120
TGGTCGC647650.09.050179120
GTGGTCG703050.08.968493120
CCGTATC458950.08.9673120
AAAAAAA1354650.08.93268120
GGTGGTC772750.08.8893385120
CGTATCA410650.08.765615120
GTCGCCG597750.08.400572120
CGCCGTA512050.08.377157120
CTCGGTG973050.08.329581120
TCGCCGT573600.08.304516120
TCGGTGG899500.08.297022120
TCTCGGT1031350.08.236832120
AGATCTC1257000.08.018202120
CGGTGGT841550.08.012895120
GCCGTAT486400.07.980199120
Produced by FastQC (version 0.11.5)