FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13232663
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC167850.083.87688120
GAGCACA272300.050.802727120
AGAGCAC371550.038.212376120
AAGAGCA558800.025.46337118-119
GAAGAGC631500.022.665052118-119
GGAAGAG791200.018.617466120
CGGAAGA945750.015.671368120
TCGGAAG962050.015.372654118-119
ATCGGAA1118800.013.385137120
GATCGGA1142250.012.981571120
AGATCGG1248500.011.977461118-119
TAGATCG288500.011.11955120
CTAGATC69650.010.710012108-109
CACGTCT49550.010.036499120
AAGATCG489350.010.0195120
GAGATCG435050.09.981547114-115
CACACGT48000.09.776899120
TTAGATC112750.09.691156120
ACACGTC48050.09.475181120
ACGTCTG65300.09.010183120
Produced by FastQC (version 0.11.5)