FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11623841
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC200350.0108.34709120
GAGCACA323950.066.78531120
AGAGCAC442900.049.297253120
AAGAGCA661300.033.085537118-119
GAAGAGC727550.029.688921118-119
GGAAGAG885500.024.510199116-117
CACGTCT57150.022.593937120
ACACGTC58400.021.801098120
CACACGT56650.021.04002120
CGGAAGA1034400.020.938454116-117
TCGGAAG1082800.020.069464114-115
ATCGGAA1273500.017.361303114-115
GATCGGA1304750.016.915161112-113
AGATCGG1409500.015.658076112-113
ACGTCTG70150.015.446342120
TAGATCG350550.014.47633120
GCACACG76850.014.217185120
CTAGATC108150.013.703705118-119
AAGATCG497150.013.102699110-111
GAGATCG450000.013.074063110-111
Produced by FastQC (version 0.11.5)