FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12353591
Sequences flagged as poor quality0
Sequence length50-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG390350.09.345723120
TAGATCT280650.09.064927120
AAGAGTG516700.08.964836120
GTGTAGA384750.08.951519120
AGAGTGT458600.08.844558120
AGATCTC284100.08.659167120
GTAGATC397250.08.609431120
GAGTGTA420200.08.346565118-119
GTAGGGA779000.07.941174120
TAGGGAA738650.07.8875847120
TGTAGGG833300.07.7909317120
GTGTAGG904750.07.646527120
ATCTCGG279000.07.613118120
TGTAGAT405850.07.3477497120
AAAGAGT596450.07.2936225118-119
GATCTCG314150.07.1050863120
GGGAAAG718200.07.0177417120
GTGGTCG189950.07.0125694120
AGGGAAA773000.07.001491120
TCGGTGG233250.06.996982120
Produced by FastQC (version 0.11.5)