FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13243236
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC178350.079.46963120
GAGCACA284300.049.28067120
AGAGCAC365200.038.53664118-119
AAGAGCA563700.024.920208120
GAAGAGC604400.023.13842116-117
GGAAGAG723700.018.965782118-119
CGGAAGA825300.017.061872114-115
TCGGAAG849850.016.124563116-117
CACGTCT51350.014.565094120
ATCGGAA1006550.014.126104112-113
ACACGTC52950.013.705424120
CACACGT52050.013.6578665120
GATCGGA1026900.013.506782114-115
AGATCGG1128350.012.610975110-111
TAGATCG293350.011.498559116-117
CTAGATC82700.010.565957120
ACGTCTG64500.010.332732120
CCCCCCG6103.935777E-59.991811
GAGATCG419050.09.74622112-113
CAGATCG424700.09.67759112-113
Produced by FastQC (version 0.11.5)