FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11603931
Sequences flagged as poor quality0
Sequence length51-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGAA122150.010.461055120
TCGTGTA197200.010.2520895120
CGTGTAG181800.010.064571120
GTAGGGA133850.09.546641120
AGCGTCG310750.09.208664120
GTCGTGT213900.09.030971120
GTGTAGG176200.08.920408120
AGAGCGT356750.08.912535120
CGTCGTG280200.08.606911120
GAGCGTC330800.08.523574120
TCGGAAG483950.08.379837120
TGTAGGG171600.08.145693120
GGAAGAG461350.08.101153120
GATCGGA556850.08.08002120
GAGTGTA69750.07.912851120
GAAGAGC418450.07.8994594120
TAGATCG165800.07.8878994120
AAGAGCG386750.07.7868676118-119
GCGTCGT294700.07.5930815118-119
ATCGGAA551900.07.4133306120
Produced by FastQC (version 0.11.5)