FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12723729
Sequences flagged as poor quality0
Sequence length62-126
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT108000.011.844032120
AGTGTAG156350.010.562788120
GAGTGTA171250.010.5204525120
GTGTAGA164850.010.382445120
AAGAGTG223600.010.017994120
TAGGGAA357200.09.919375120
AGAGTGT192600.09.821957120
AGATCTC123550.09.672842120
GTAGGGA379700.09.426477120
TGTAGGG429700.09.000451120
GTGTAGG466150.08.760462120
AAAGAGT271450.08.694537120
GTAGATC184200.08.541918120
CGTGTAG492050.08.445796120
GGGAAAG380000.08.423399120
AGGGAAA399950.08.393631120
TCGTGTA523900.08.367933120
GTCGTGT562400.07.997979120
TGTAGAT191050.07.8270135120
CGTCGTG646000.07.818208120
Produced by FastQC (version 0.11.5)