FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12649979
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC150050.071.05619120
GAGCACA238750.043.8394120
AGAGCAC318150.035.057858120
AAGAGCA487250.023.112207120
GAAGAGC544900.021.124296120
GGAAGAG648150.018.029387120
CGGAAGA775600.015.249082120
TCGGAAG784250.014.952067120
GATCGGA933250.013.005103120
ATCGGAA934200.012.934199120
AGATCGG1030350.011.975612120
CTAGATC56850.011.018171120
TAGATCG217100.010.905248114-115
GAGATCG324000.09.749556120
TTAGATC94300.09.71369120
AAGATCG441150.09.557517120
CCTAGAT16650.09.304019120
CAGATCG338700.08.8789015118-119
TAAGATC179150.08.383803118-119
CACGTCT44650.08.296564120
Produced by FastQC (version 0.11.5)