FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14467494
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT329750.010.1347685120
AGTGTAG473700.09.993487120
GAGTGTA516100.09.718871120
AGATCTC328500.09.497546120
AGAGTGT566650.09.307166120
AAGAGTG637950.08.925303120
GTGTAGA464450.08.874853120
ATCTCGG313150.08.851829120
GGTGGTC212300.08.506678120
GTAGATC497750.08.353441120
CTCGGTG271350.08.291724120
TAGGGAA940800.08.290668120
GATCTCG342350.08.026729120
GTAGGGA993750.07.993818120
TGTAGAT496150.07.957128120
TCTCGGT276900.07.930518120
AAAGAGT735150.07.8349953120
GGGAAAG916350.07.758905120
TGTAGGG1063750.07.744161120
GTGTAGG1153800.07.477765120
Produced by FastQC (version 0.11.5)