FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14655444
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG81200.010.954286120
TAGATCT608350.010.304079120
GATCTCG551050.09.543232120
ATCTCGG508250.09.495472120
AGATCTC566650.09.460811120
TCTCGGT462050.09.287261120
TCGGTGG420650.09.2726145120
GTAGATC795450.08.968426120
GTGGTCG310700.08.93674120
GGTGGTC340500.08.93124120
AGTGTAG851300.08.881297120
CTCGGTG427200.08.863142120
TGGTCGC280700.08.735669120
GTGTAGA815450.08.696872120
GGTCGCC283450.08.566103120
CGGTGGT399400.08.562145120
TGTAGAT824700.08.4973120
GAGTGTA921950.08.441912120
AGAGTGT988850.08.277993120
AAGAGTG1084350.07.920291120
Produced by FastQC (version 0.11.5)