FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19511625
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA1789400.011.278835120
CGTATCA656600.08.059042120
GGTCGCC976950.07.977237120
TGGTCGC1018600.07.93377120
CGCCGTA835700.07.889745120
GCCGTAT795050.07.885651120
CCGTATC748950.07.86637120
TCGCCGT947600.07.6545024120
TCATTAA555150.07.618939120
GTATCAT688950.07.576127120
GTGGTCG1093500.07.2696385118-119
ATCATTA637050.07.195079120
GTCGCCG987150.07.147265120
GGTGGTC1170750.07.015441120
CATTAAA580500.07.0071783120
TCGGTGG1342400.06.9094462120
CGGTGGT1282850.06.7110696120
CTCGGTG1425300.06.6885705120
TCTCGGT1479800.06.584135120
TATCATT763000.06.5656486120
Produced by FastQC (version 0.11.5)