FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19511625
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC233500.076.15246120
GAGCACA365750.046.91503120
AGAGCAC482250.036.344746118-119
AAGAGCA736050.023.495468118-119
GAAGAGC803700.021.781801116-117
GGAAGAG981400.017.628807116-117
CGGAAGA1135300.015.497619114-115
TCGGAAG1147650.014.9795475114-115
GATCGGA1393300.012.929741120
ATCGGAA1378700.012.753823112-113
AGATCGG1504150.011.821884118-119
TAGATCG380700.011.100961110-111
CTAGATC93200.09.865816110-111
CACGTCT70550.09.123258120
GAGATCG561950.08.886271118-119
AAGATCG559650.08.771129114-115
TTAGATC156100.08.699692120
CAGATCG505500.08.024627116-117
CACACGT64100.07.9447455120
ACACGTC72700.07.7832522120
Produced by FastQC (version 0.11.5)