FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14714785
Sequences flagged as poor quality0
Sequence length60-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA367500.08.031691120
TAGATCT269750.08.028695120
AGTGTAG359950.08.016819120
GTAGATC377700.07.624185120
GAGTGTA386150.07.5661006120
AGAGTGT419500.07.536641120
AAGAGTG475450.07.4068317120
AGATCTC287750.07.2137313120
ATCTCGG280550.07.1636395120
TAGGGAA636550.07.049662120
TCGGTGG242300.06.8584437120
TCTCGGT253550.06.719763120
TGTAGGG708150.06.6588078120
GTAGGGA664250.06.611176120
GTGTAGG758550.06.5801883120
GTCGTGT882550.06.4985695120
CTCGGTG260050.06.4364524120
GTGGTCG194750.06.407448120
TGGTCGC174000.06.3957853118-119
TCGTGTA836750.06.395409120
Produced by FastQC (version 0.11.5)