FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13326523
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC114650.065.51521120
GAGCACA182250.045.74304120
AGAGCAC243050.033.37888120
AAGAGCA405200.020.763685120
GAAGAGC438100.019.773956120
GGAAGAG557300.016.302246120
TCGGAAG647750.015.043214120
CGGAAGA657300.014.503431120
ATCGGAA782850.012.962022120
GATCGGA789250.012.694783120
AGATCGG850400.011.778078118-119
TAGATCG213250.010.920953120
GAGATCG312950.09.220399120
CAGATCG358350.09.177066120
CTAGATC57900.09.061115114-115
CCTACCC20450.08.17767510-11
AAGATCG290850.07.985198120
TTAGATC100000.07.357766120
ATCCTAC52000.07.33480179
CCCTCCC31750.07.2074982
Produced by FastQC (version 0.11.5)