FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11768743
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC108500.063.16545120
GAGCACA161850.042.81759120
AGAGCAC216650.033.57772120
AAGAGCA344850.021.391079120
GAAGAGC395400.019.463266120
GGAAGAG498650.015.778739120
TCGGAAG587200.013.8665905120
CGGAAGA602050.013.609354120
GATCGGA664250.012.565542120
ATCGGAA682050.012.172118120
AGATCGG737150.011.669147120
CTAGATC47550.011.004451120
TAGATCG184100.010.779823120
TTAGATC81150.010.14393120
GAGATCG260100.09.322842120
AAGATCG308250.09.129365120
CAGATCG239600.08.442625118-119
CCTAGAT18250.08.04211118-119
TGAGATC109200.07.538278120
ATAGATC81700.07.185729120
Produced by FastQC (version 0.11.5)