FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15655236
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC161600.068.59383120
GAGCACA259700.044.21555120
AGAGCAC335850.034.1902120
AAGAGCA543850.021.498486118-119
GAAGAGC590650.019.541996120
GGAAGAG709950.016.968287120
TCGGAAG830950.015.207927120
CGGAAGA818250.014.649474120
ATCGGAA985000.012.896815120
GATCGGA982450.012.849499118-119
AGATCGG1086400.011.552853118-119
TAGATCG277550.011.233279120
CTAGATC71200.09.96954114-115
GAGATCG433050.09.252738114-115
AAGATCG341500.09.246261120
TTAGATC103650.08.864175118-119
ACACGTC56550.08.797891120
CAGATCG345500.08.486412120
ATAGATC140450.07.3920336114-115
TAAGATC149850.07.348543120
Produced by FastQC (version 0.11.5)