FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14514708
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC112650.055.575314120
GAGCACA183650.038.644104120
AGAGCAC238700.028.829266120
AAGAGCA407900.018.253256120
GAAGAGC446050.016.146372118-119
GGAAGAG553950.014.882055120
TCGGAAG585100.014.013944120
CGGAAGA607750.012.84523120
GATCGGA681350.012.275512118-119
ATCGGAA709200.012.008443120
AGATCGG754800.011.434085120
TAGATCG227100.010.817449116-117
CTAGATC57050.010.107457120
TTAGATC86800.08.899501114-115
GAGATCG281450.08.615977118-119
AAGATCG275800.08.385999120
CAGATCG280000.07.3265114116-117
ATAGATC127800.06.842335120
CCTAGAT23550.06.452765114-115
TCTAGAT39850.06.275897118-119
Produced by FastQC (version 0.11.5)