FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14726818
Sequences flagged as poor quality0
Sequence length51-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG88150.011.864346120
AAAAAAA1859650.011.852133120
GTATCAT682050.09.253157120
CCGTATC757500.09.2202120
TCTCGGG32650.09.204573120
GTCGCCG1055600.08.894028120
CGTATCA678500.08.858639120
GGTCGCC1074200.08.79598120
CGCCGTA881900.08.764729120
GTGGTCG1229650.08.514977120
TGGTCGC1140400.08.480387120
GCCGTAT819700.08.469229120
GGTGGTC1328000.08.386759120
TCATTAA606550.08.046486120
ATCATTA677600.07.9922347120
TCGCCGT990550.07.979326120
CGGTGGT1445100.07.948398120
TCGGTGG1537650.07.9038825120
CTCGGTG1644650.07.5321913120
ATCTCGG1867400.07.444835120
Produced by FastQC (version 0.11.5)