FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12814614
Sequences flagged as poor quality0
Sequence length50-126
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC141300.059.725975120
GAGCACA213050.039.12719120
AGAGCAC284800.031.875116120
AAGAGCA432150.022.066711120
GAAGAGC503350.019.086323120
GGAAGAG593300.017.334513120
TCGGAAG701100.014.595565120
CGGAAGA712500.014.094958118-119
ATCGGAA831150.012.800854120
GATCGGA847800.012.313588118-119
AGATCGG970300.011.463807120
GCCTAGA6902.978013E-711.220956120
TAGATCG182650.011.056619120
CACACGT37000.010.8115425120
CACGTCT43950.010.423106120
CCTAGAT18400.010.168991120
TTAGATC63450.010.168759118-119
TCTAGAT19200.010.081327120
GCTAGAT21600.09.857298120
CTAGATC53400.09.786817120
Produced by FastQC (version 0.11.5)