FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13482245
Sequences flagged as poor quality0
Sequence length54-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG41350.09.150592120
AGTGTAG411100.08.195953120
TAGATCT305750.08.171674120
TCTCGGT269400.07.8697553120
GAGTGTA445000.07.8010306120
GGTGGTC206450.07.6511197120
GATCTCG287600.07.64322120
ATCTCGG283600.07.623956120
GTGGTCG178850.07.455009120
GTGTAGA410600.07.4160585120
AGATCTC305250.07.3193316120
GTAGGGA727450.07.166394120
GTAGATC409350.07.130593120
AGAGTGT481950.07.058342118-119
TAGGGAA697450.06.863243120
TGGTCGC166850.06.839298120
TGTAGGG780700.06.685282120
TCGGTGG238950.06.6607475120
AAGAGTG552000.06.6479278120
CGCCGTA142100.06.5934796120
Produced by FastQC (version 0.11.5)