FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13482245
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC131600.051.23576120
GAGCACA177900.034.68015120
AGAGCAC222950.030.1571120
AAGAGCA387600.017.855783120
GAAGAGC404700.017.840746120
GGAAGAG499600.014.286188120
TCGGAAG545050.013.305173120
CGGAAGA559150.012.968581118-119
GATCGGA617300.011.892309120
ATCGGAA638800.011.641557120
TAGATCG190200.011.548929120
AGATCGG684250.010.755458118-119
CTAGATC50300.09.873241120
AAGATCG288000.08.445086120
GAGATCG240100.08.326656120
TTAGATC87000.07.998462112-113
ACACGTC35350.07.5647225120
CAGATCG212450.07.55226120
ATAGATC109950.07.0068574120
TAAGATC120350.06.612978120
Produced by FastQC (version 0.11.5)