FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11838336
Sequences flagged as poor quality0
Sequence length50-126
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC175100.0105.9301120
GAGCACA286250.063.17999120
CGATCTA37400.060.8546755
CCGATCT121150.058.7516144
TCCGATC130300.055.0378843
CTTCCGA140100.051.7787931
AGAGCAC397350.046.70097120
TTCCGAT175500.041.658122
GATCTAG10150.036.4907466
AAGAGCA635550.029.111746118-119
GAAGAGC690950.026.87311118-119
GATCTAT46450.023.2279036
GGAAGAG826700.022.48664120
CGATCTC70450.022.019965
CGATCTT86700.021.050365
GATCTAA22100.020.6455446
CGATCTG79950.020.6119775
CACACGT39150.019.643887120
CGGAAGA964150.019.262667116-117
TCGGAAG1013150.018.217037116-117
Produced by FastQC (version 0.11.5)