FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11565539
Sequences flagged as poor quality0
Sequence length60-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA845350.013.5974655120
GCGTCGG81750.09.46675120
ATCATTA357500.09.330346120
GTGGTCG714600.09.251616120
CGTATCA404750.09.189753120
GTATCAT405650.09.110207120
TCATTAA298000.08.998918120
CATTAAA295000.08.846394120
GCCGTAT498700.08.757719120
CGCCGTA535150.08.710528120
TATCATT429750.08.557435120
GGTCGCC632950.08.492539120
TGGTCGC659650.08.458013120
GGTGGTC778550.08.18346120
GTCGCCG626950.08.104934120
TCGCCGT597300.08.045239120
CCGTATC483300.07.956833120
TCGGTGG897500.07.7071505120
CGGTGGT861400.07.5913777118-119
CTCGGTG950150.07.482129120
Produced by FastQC (version 0.11.5)