FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11565539
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC157400.085.41462120
GAGCACA242300.052.734642120
AGAGCAC333150.039.95476120
AAGAGCA487750.027.078644118-119
GAAGAGC559900.023.31855116-117
GGAAGAG705900.018.877665120
TCGGAAG820000.016.115873118-119
CGGAAGA822300.016.012613114-115
ATCGGAA959450.013.950705120
GATCGGA985800.013.533427116-117
CACGTCT45250.012.441120
AGATCGG1079000.012.327626112-113
TAGATCG219600.011.823774110-111
CACACGT41100.011.354593118-119
CTAGATC54000.011.3173065114-115
ACACGTC46850.010.593466118-119
AAGATCG430150.09.695525114-115
ACGTCTG59000.09.667163120
TTAGATC81550.09.629359112-113
TCTAGAT26700.09.432525120
Produced by FastQC (version 0.11.5)