FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11847726
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC133000.072.51762120
GAGCACA221600.045.52098120
AGAGCAC289350.034.360172120
AAGAGCA444350.023.50439120
GAAGAGC513600.021.261316120
GGAAGAG617800.017.413343118-119
TCGGAAG736000.015.159823120
CGGAAGA726700.014.835675118-119
ATCGGAA864700.012.811792120
GATCGGA868900.012.784078118-119
TAGATCG225050.011.800199120
AGATCGG963950.011.492668118-119
CTAGATC54400.010.686269120
AAGATCG304200.09.381382120
CAGATCG348050.09.319276120
GAGATCG385750.09.221936118-119
TTAGATC74900.08.775694116-117
GCTAGAT20500.08.539536118-119
ATAGATC106800.07.9792256118-119
TCTAGAT26250.07.675572116-117
Produced by FastQC (version 0.11.5)