FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15196753
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC178300.074.60244120
GAGCACA276850.047.99582120
AGAGCAC371050.036.58287120
AAGAGCA565650.024.094028118-119
GAAGAGC642350.020.8204116-117
GGAAGAG798300.017.44129120
TCGGAAG928150.014.82295118-119
CGGAAGA931150.014.72272118-119
ATCGGAA1086750.012.895515120
GATCGGA1086150.012.695605118-119
AGATCGG1187450.011.583143114-115
CTAGATC65300.011.447431120
TAGATCG282500.010.6987915114-115
TTAGATC90550.010.261258120
AAGATCG417900.09.227926120
GAGATCG436650.09.071907118-119
CCTAGAT22850.09.011601118-119
CCCTAGA8701.513315E-48.833054120
ACACGTC64500.08.55667120
CAGATCG419500.08.502769110-111
Produced by FastQC (version 0.11.5)