FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14522756
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC160850.071.86643120
GAGCACA253600.047.026993120
AGAGCAC342650.036.626766120
AAGAGCA534950.024.32277120
GAAGAGC590650.021.614918118-119
GGAAGAG729000.017.66226120
CGGAAGA861900.015.096257120
TCGGAAG869250.014.862654118-119
ATCGGAA1027300.012.903421120
GATCGGA1042500.012.464219116-117
AGATCGG1146800.011.78955120
TAGATCG276250.011.151923118-119
CTAGATC69450.010.443573114-115
TTAGATC110050.09.023325116-117
CTCTAGA12051.5074693E-79.007629120
GAGATCG430800.08.9531145114-115
CAGATCG487100.08.620676118-119
ACACGTC53600.08.606403120
CCCTAGA8305.0931703E-108.574905118-119
CCTAGAT23250.08.461602120
Produced by FastQC (version 0.11.5)