FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13036609
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC148650.071.73283120
GAGCACA235750.045.51387120
AGAGCAC312300.034.265682118-119
AAGAGCA488250.022.149551118-119
GAAGAGC526900.020.47405116-117
GGAAGAG644550.017.7044120
CGGAAGA743500.015.393109120
TCGGAAG757800.014.970409118-119
GATCGGA904300.012.715056120
ATCGGAA897950.012.529909118-119
AGATCGG995050.011.588997120
TAGATCG250350.011.18192120
AAGATCG380400.09.263195118-119
CTAGATC61700.09.239262118-119
GAGATCG361150.09.046305120
TTAGATC107300.08.792513118-119
CAGATCG318250.07.960737114-115
ATAGATC134550.07.29934120
TAAGATC153850.07.1072206116-117
GCTAGAT26500.07.0593076120
Produced by FastQC (version 0.11.5)