FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17065176
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG65750.020.531921120
TGGTCGC503900.09.6207695120
GTCGCCG455050.09.00543120
CGCCGTA389250.08.631818120
AAAAAAA832750.08.581041120
GGTCGCC475950.08.521739120
GGTGGTC595250.08.466867120
GTGGTCG536300.08.329487118-119
TCGGTGG702350.08.252136120
CTCGGTG742450.08.234739120
TAGATCT983200.08.207733120
GATCTCG875950.08.061948120
ATCTCGG859450.08.049178120
AGATCTC932100.08.039755120
CGGTGGT672850.07.9540706120
TCTCGGT814950.07.619952120
GCCGTAT402000.07.611793120
TCGCCGT467300.07.5239286120
CCGTATC390400.07.4230123120
CGTATCA353750.07.1744194120
Produced by FastQC (version 0.11.5)