FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17065176
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC185250.082.47792120
GAGCACA325000.046.525486120
AGAGCAC409400.037.30122120
AAGAGCA661400.024.333263120
GAAGAGC742500.021.973831120
GGAAGAG887150.018.49772118-119
CGGAAGA965900.016.731558118-119
TCGGAAG1010650.016.214104120
ATCGGAA1186450.013.814717118-119
GATCGGA1203400.013.7394905114-115
AGATCGG1339550.012.389533114-115
TAGATCG314750.011.941021120
AAGATCG578200.011.06567114-115
GAGATCG362800.010.32915112-113
CTAGATC83400.010.246232118-119
ACACGTC49000.010.011732120
TTAGATC122250.09.99456112-113
CAGATCG356150.09.8703785112-113
CACACGT53750.08.979765120
TAAGATC244950.08.917273112-113
Produced by FastQC (version 0.11.5)