FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13589029
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC155100.059.798447120
GAGCACA248350.040.069084120
AGAGCAC322900.032.046772120
AAGAGCA501900.020.565752118-119
GAAGAGC571150.018.055143118-119
GGAAGAG685800.016.217384120
CGGAAGA768450.014.413891120
TCGGAAG778500.014.156888118-119
ATCGGAA917550.012.674183120
GATCGGA930650.012.53771120
AGATCGG1031600.011.342393118-119
TAGATCG225050.010.924335120
ACACGTC63600.010.533248120
CTAGATC64050.09.443784120
CACACGT52700.09.132786120
CACGTCT60950.09.070419120
AAGATCG473550.08.99617120
TTAGATC90500.08.983515118-119
GAGATCG357650.08.274378120
CAGATCG328250.08.08426118-119
Produced by FastQC (version 0.11.5)