FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11559610
Sequences flagged as poor quality0
Sequence length60-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT205500.010.363399120
GAGTGTA305250.09.295893120
AGTGTAG284900.08.991271120
GTGTAGA287300.08.916161120
GATCTCG214800.08.713767120
AGATCTC209900.08.68854120
ATCTCGG186850.08.604533120
AAGAGTG384700.08.452064120
AGAGTGT333650.08.4327135120
GTAGATC292300.07.983741120
TCTCGGT165750.07.745436120
CTCGGTG168900.07.707539120
TAGGGAA538900.07.6922874120
TCGGTGG173950.07.5872407120
GTAGGGA562900.07.4069467120
GGTGGTC137450.07.3761144120
TGTAGAT318550.07.0621886120
GTGTAGG647350.07.00597120
TGTAGGG594850.06.988942118-119
GGGAAAG549600.06.8876057120
Produced by FastQC (version 0.11.5)