FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14936784
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC173350.074.96198120
GAGCACA267000.046.238182120
AGAGCAC356800.035.64039118-119
AAGAGCA555500.023.332497120
GAAGAGC627450.021.520763120
GGAAGAG765300.017.49388118-119
CGGAAGA923350.014.798068120
TCGGAAG928250.014.518114120
ATCGGAA1057150.012.819167118-119
GATCGGA1070850.012.501843116-117
AGATCGG1184750.011.412738116-117
TAGATCG247450.011.357362120
CTAGATC60150.010.2207118-119
TTAGATC88100.09.177466116-117
GAGATCG473450.09.060558112-113
AAGATCG388850.09.012753112-113
CAGATCG427250.08.406394116-117
TAAGATC147650.08.326806112-113
CCTAGAT22500.07.572571110-111
TGAGATC176050.07.5530148114-115
Produced by FastQC (version 0.11.5)