FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16627639
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC128050.055.865456120
GAGCACA195750.039.072456120
AGAGCAC259250.029.840425120
AAGAGCA424150.019.420618120
GAAGAGC487950.016.560415120
GGAAGAG627450.014.196369120
TCGGAAG680750.013.581743120
GATCGGA782650.012.88591120
ATCGGAA814100.012.249606120
CGGAAGA738500.012.129484120
AGATCGG866150.011.636429120
TAGATCG211300.010.579104118-119
CTAGATC54200.09.979051118-119
TTAGATC90250.08.833551118-119
AAGATCG363700.08.801048120
GAGATCG304650.08.743409118-119
CAGATCG293850.08.096575118-119
CCTAGAT22850.07.114793116-117
TGAGATC137300.06.9905763116-117
TCTAGAT28800.06.7425785120
Produced by FastQC (version 0.11.5)