FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15752311
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC203250.083.955284120
GAGCACA315500.051.50646120
AGAGCAC432150.039.62301120
AAGAGCA640650.026.491325118-119
GAAGAGC747000.022.937613118-119
GGAAGAG899250.018.977438116-117
CGGAAGA1056200.016.290482116-117
TCGGAAG1056850.016.123285114-115
ATCGGAA1218550.014.141583114-115
GATCGGA1232350.013.827945112-113
AGATCGG1352350.012.767776112-113
CTAGATC84550.012.206049110-111
TAGATCG289600.012.172474110-111
CACGTCT62150.011.782164120
AAGATCG558700.010.632892110-111
TTAGATC113100.010.36767110-111
GAGATCG402400.09.909352110-111
CAGATCG437200.09.64436114-115
CACACGT55950.09.518508118-119
CCTAGAT24200.09.47692108-109
Produced by FastQC (version 0.11.5)