FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13830483
Sequences flagged as poor quality0
Sequence length52-126
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA2418900.013.2846775120
GCGTCGG59200.013.174981120
CGTATCA875950.08.719216120
TCTCGGG17900.08.714624120
CCGTATC961000.08.659273120
GCCGTAT1026050.08.285709120
CGCCGTA1085600.08.278857120
GTATCAT902900.08.133362120
GTCGCCG1240600.07.9747534120
GGTCGCC1304950.07.91253120
TCATTAA849150.07.8144627120
CATTAAA790900.07.790714120
TCGCCGT1181250.07.745624120
GGTGGTC1590250.07.560677120
TGGTCGC1390550.07.5074253120
GTGGTCG1482750.07.2388706120
CGGTGGT1705800.07.0168657120
TCGGTGG1802950.06.9316072120
ATCATTA977200.06.815032120
CCCCCAC11553.0153524E-46.75281761
Produced by FastQC (version 0.11.5)