FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13830483
Sequences flagged as poor quality0
Sequence length50-126
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC187400.0101.543785120
GAGCACA303850.060.114647120
AGAGCAC415000.044.964954118-119
AAGAGCA650750.028.951962120
GAAGAGC697100.027.686436120
GGAAGAG847400.022.63118118-119
CGGAAGA972700.019.428349120
TCGGAAG1029800.018.65402120
CACGTCT47550.018.127932120
ACACGTC44900.017.369476120
CACACGT44850.016.656681120
ATCGGAA1217650.015.840753118-119
GATCGGA1259700.015.258667114-115
AGATCGG1372250.014.113533116-117
TAGATCG360750.013.176901116-117
GAGATCG497550.012.583739112-113
ACGTCTG58000.012.455586120
GCACACG60850.011.8722105120
CGTCTGA73100.011.230319120
AAGATCG393500.011.12048116-117
Produced by FastQC (version 0.11.5)