FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18401808
Sequences flagged as poor quality0
Sequence length52-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT200600.09.00218120
GTAGATC278300.08.148746120
AGAGTGT348150.08.081995120
TAGGGAA520350.08.013115120
GTGTAGA293900.07.961175120
GAGTGTA306400.07.7930317120
AGTGTAG280650.07.7598567118-119
TGTAGGG570050.07.546027120
TCGTGTA709750.07.480834120
GTAGGGA530750.07.4265566120
AAGAGTG396100.07.2399435120
CGTGTAG657000.07.1774354120
AGATCTC221750.07.1425357120
GTCGTGT735450.07.1133714120
GTGTAGG622650.07.00491120
ATCTCGG219250.06.92298120
CGTCGTG814250.06.727058120
AGCGTCG897600.06.543528120
GAGCGTC974000.06.504548120
GCGTCGT851800.06.3037295118-119
Produced by FastQC (version 0.11.5)